The Doubleedge Sword Of CRISPR Application For In Vivo Studies
A new editorial titled "The Double-Edged Sword of CRISPR Applications in Vivo Studies" has been published in the journal OncoTarget .
In this new article, Martin K., a researcher at Aarhus University. Thomsen wrote his editorial analyzing an important paper published a decade earlier by Platt and others. has been published. reported the in vivo application of regularly interspaced short palindromic repeats (CRISPR) to induce cancer in various organs in mice.
This signature article presents advances in the delivery of sgRNAs to target tissues to generate loss- or gain-of-function mutations without the need for timely genetic crossing of mouse strains. In addition, this study shows that multiplexing is possible, allowing the method to address multiple sites simultaneously. This technology was expected to change the way mouse cancer models are made, but even 10 years later, few studies have relied on this approach.
"The double-edged sword of using CRISPR in vivo is the lack of variation in the target sequence," Thomsen wrote.
When CRISPR causes mutations, they do not always occur, resulting in cells without the desired mutation. This is further complicated by the presence of various types of indels, which can produce functional proteins with only a few amino acid changes without the need to introduce a premature stop codon. This results in variation from clone to clone and produces tumors with different mutational profiles. However, this is an advantage of CRISPR in developing in vivo cancer models, where natural selection would lead to the Darwinian evolution of cancer.
"Overall, applications of CRISPR in vivo will become more common. Although this technology presents challenges, it will become more practical in the future, allowing more researchers to adopt this technology."
More information: Martin K. Thomsen, The double-edged sword of CRISPR application in vivo studies, Oncotarget (2023). doi:10.18632/oncotarget.28459
Provided by Impact Journals LLC
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